Research
Summary
Molecules of biological origin, including
proteins and DNA, are gaining recognition as having
potential as active elements in a wide variety of technologies,
from sensors to microelectronics to optical memories.
This burgeoning field is quickly becoming rife with
examples of how natural materials can help solve problems
in a more facile and efficient manner than conventional
technologies, particularly in the field of nanotechnology.
Bio-nanotechnology is bridging the gap between nano-
and biotechnology, and central to this field is the
need to understand how biology works at the molecular
level, and how we can probe—and manipulate—biological
events at this scale. Furthermore, the interface between
biological and synthetic systems must be understood
with respect to signal transduction and stability.
Our work seeks to understand and exploit
the properties of biological molecules, specifically
the photoactive proteins bacteriorhodopsin & proteorhodopsin,
and DNA, through basic research and a number of collaborations.
Current projects include:
• Protein-based holography
• Protein-based chemical sensing (development
of a biophotonic artificial nose)
• Development and evaluation of advanced functional
biomaterials
Selected publications
1. Xi, B., W. Tetley, D.L. Marcy, C.
Zhong, G. Whited, R.R. Birge, and J.A. Stuart, Evaluation
of blue and green absorbing proteorhodopsins as holographic
materials. J. Phys. Chem. B, 2007. Submitted Manuscript.
2. Yordy, B., J. Girard, J.F. Koscielecki, J.R. Hillebrecht,
W. Tetley, D.L. Marcy, and J.A. Stuart, Utilization
of bacteriorhodopsin mutants in chemical sensor architectures.
Manuscript in preparation, 2007.
3. Ner, Y., J.G. Grote, J.A. Stuart, and G.A. Sotzing,
Enhanced fluorescence in electrospun dye doped DNA nanofibers.
Advanced Materials, 2007. Submitted Manuscript.
4. Stuart, J.A., R.R. Birge, M.P. Krebs, B. Xi, W. Tetley,
D.L. Marcy, J.F. Koscielecki, and J.R. Hillebrecht,
Protein-based optical memories, in Nano and Molecular
Electronics Handbook, S. Lyshevski, Editor. 2007, Taylor
& Francis Group, LLC.: Boca Raton, FL. p. 16-1 –
16-23.
5. Stuart, J.A., R.R. Birge, P. Bhattacharya, B.J. Yordy,
J. Girard, W. Tetley, D.L. Marcy, J.F. Koscielecki,
and J.R. Hillebrecht, Bacteriorhodopsin: from biophotonic
material to chemical sensor, in Smart Biosensor Technology,
G.K. Knopf and A.S. Bassi, Editors. 2006, Marcel Dekker,
Inc: New York. p. 355-384.
6. Lee, I., E. Greenbaum, S. Budy, J.R. Hillebrecht,
R.R. Birge, and J.A. Stuart, Photoinduced surface potential
change of bacteriorhodopsin mutant D96N measured by
scanning surface potential microscopy. J. Phys. Chem.
B, 2006. 110(22): p. 10982-10990.
7. Hillebrecht, J.R., J. Galan, R. Rangarajan, L. Ramos,
K. McCleary, D.E. Ward, J.A. Stuart, and R.R. Birge,
Structure, function, and wavelength selection in blue-absorbing
proteorhodopsin. Biochemistry, 2006. 45(6): p. 1579-1590.
8. Hillebrecht, J.R., J.F. Koscielecki, K.J. Wise, D.L.
Marcy, W. Tetley, R. Rangarajan, J. Sullivan, M. Brideau,
M.P. Krebs, J.A. Stuart, and R.R. Birge, Optimization
of protein-based volumetric optical memories and associative
processors using directed evolution. Nanobiotechnology,
2005. 1(2): p. 141-152.
9. Stuart, J.A., D.L. Marcy, K.J. Wise, and R.R. Birge,
Biomolecular electronic device applications of bacteriorhodopsin,
in Molecular Electronics: Bio-sensors and Bio-computers,
L.E.A. Barasanti, Editor. 2003, Kluwer Academic Publishers.
p. 265-299.
10. Birge, R.R., N.B. Gillespie, E.W. Izaguirre, A.
Kusnetzow, A.F. Lawrence, D. Singh, Q.W. Song, E. Schmidt,
J.A. Stuart, S. Seetharaman, and K.J. Wise, Biomolecular
Electronics: Protein-based associative processors and
volumetric memories. J. Phys. Chem. B, 1999. 103: p.
10746-10766.
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