The Nuclear Magnetic Resonance (NMR) Facility at UConn recognizes the important role that NMR plays in molecular structure determination, drug design, protein and nucleic acid characterization, and conformational analysis. The instrumentation serves the high-resolution research needs for investigators from the Departments of Molecular and Cell Biology, Chemistry, and Pharmacy at the University of Connecticut. The Facility is part of the University-wide Partnership for Excellence in Structural Biology and maintains collaborative ties with its sister NMR Facility at the UConn Health Center in Farmington. The services and equipment of the UConn NMR Facility are also available to industry and other academic institutions.

The NMR Facility directly supports and impacts research programs in the following areas:

Chemical synthesis
Pharmaceutical chemistry
Molecular recognition & drug binding
Macromolecules
Nanomaterials
Analysis of Chemical Mixtures
Protein structure-function relationships
Protein folding and design
Nucleic acid structure and reactivity
Molecular dynamics

Instrumentation

UConn's Center for Open Research Resources and Equipment NMR Facility and the Department of Chemistry's NMR Facility offers several solution NMR instruments to the UConn faculty, researchers from other academic institutions, and industry:

Varian INOVA 600 MHz: Fitted with a triple-resonance 1H,13C,15N cryogenic probe, the Varian 600 is primarily used to characterize proteins and nucleic acids and is capable of carrying out all necessary NMR experiments for biomolecular structure determination. In addition, due to superior sensitivity of the cryoprobe (~4000 for 1H), the 600 is used to acquire 1H 1D, 1H-1H, 1H-13C and 1H-15N 2D correlation experiments (COSY, NOESY, HSQC etc.) on the natural products and low concentration samples. The Varian 600 can be found in the Chemistry Building, Room R002 (*Rates).

Bruker AVANCE 500 MHz: The spectrometer is equipped with the 5 mm BBFO SmartProbe that has the ability to observe 19F with 1H decoupling and vise-versa, as well as to perform 1H-19F 2D correlation spectroscopy. The probe has a signal-to-noise ratio 730 for 1H, 550 for 19F, 300 for 13C, 180 for 31P and 40 for 15N. Additionally, any nuclei can be automatically selected and optimally tuned and matched with the auto tune-match (ATM) component. The 500 is an instrument of choice for users with small quantities of a compound or where a higher resolution is necessary, and for multidimensional experiments such as HSQC, HMBC or NOESY. It is used for chemical structure elucidation and supports NMR studies on proteins and nucleic acids. This spectrometer is located in the Pharmacy/Biology Building, Room 056 (*Rates).

Bruker AVANCE III 400 MHz: This work horse of the Chemistry Department is equipped with the newest generation of the console and a 5mm BBFO probe with 19F capability. It delivers signal-to-noise ratio 300 for 1H, 275 for 19F, 190 for 13C, 150 for 31P, and 20 for 15N with automatic tuning and matching to selected nuclei. The AVANCE 400 is used for both one-dimensional and advanced two-dimensional experiments. It is located in the Chemistry Building, Room R002 (**Rates).

Bruker AVANCE 300 MHz: The instrument has a 5mm BBO probe with ATM component and can be used for a broad range of nuclei. It is mostly used for routine NMR spectroscopy and for undergraduate research and education (**Rates).

In addition to the above NMR spectrometers, the Institute of Materials Science houses two Bruker NMR instruments: solid-state AVANCE III 400 MHz and the solution DMX 500 MHz. Visit the IMS NMR Laboratory for more information.

Training and Education

Only trained users can be granted an access to the NMR spectrometers. The NMR Facility Staff provides training and consultation in the use of instruments under their respective supervision. A 24 hour notice is required for scheduling a hands-on training session. For specific questions regarding applications for any NMR instrument and for any general managerial questions, please contact the NMR Facility Director Dr. Vitaliy Gorbatyuk at (860) 486-4069 or at vitaliy.gorbatyuk@uconn.edu.

*NMR Service Rates: Varian INOVA 600 & Bruker AVANCE 500

Internal Users (any UConn faculty and members of their group)

Varian INOVA 600

Assisted Use/Set-up Fee: $20/hr
$8/hr flat rate, first 24 hours
$6/hr flat rate, over 24 hours

Bruker AVANCE 500

Assisted Use/Set-up Fee: $20/hr
$6/hr flat rate

External Users

Varian INOVA 600

Assisted Use/Set-up Fee: $50/hr
$50/hr flat rate, first 1 hour
$15/hr flat rate, over 1 hour

Bruker AVANCE 500

Assisted Use/Set-up Fee: $50/hr
$11/hr flat rate (non-profit)
$70/hr flat rate (for-profit)

**NMR Service Rates: Bruker AVANCE III 400 & AVANCE 300

Internal Users (any UConn faculty and members of their group)

Magnet time for Chemistry-300 (i.e. users run their samples, after the NMR training session):

$5/hr daytime
$2/hr nighttime, weekends & holidays

Magnet time for Chemistry-400 (i.e. users run their samples, after the NMR training session):

$6/hr daytime
$2/hr nighttime, weekends & holidays

Daytime: 8:30 AM to 5 PM

Nighttime: 5 PM to 8:30 AM

Outside Institutions (non-profit)

Magnet time for Chemistry 300 and 400 (i.e. users run their samples, after the NMR training session):

$10/hr flat rate, first 16 hours
$5/hr flat rate, 16-64 hours

Outside Companies (for-profit)

Magnet time for Chemistry 300 and 400 (i.e. users run their samples, after the NMR training session):

$40/hr flat rate, first 16 hours
$20/hr flat rate, 16-64 hours

Services done by the NMR Facility Manager (both non-profit and for-profit organizations)

Run samples only, any nuclei 1D (from sample prep to sending results): $50/hr
Data interpretation (technical consulting): $50/hr