The Nuclear Magnetic Resonance Facility recognizes the important role that NMR plays in molecular structure determination and conformational analysis for research projects engaging chemical synthesis, chemical mixture characterization, drug design, intermolecular interaction studies, protein and nucleic acid characterization. UConn's Center for Open Research Resources and Equipment NMR Facility and the Department of Chemistry's NMR Facility offers several solution-state NMR instruments on a fee-for-use basis. The instrumentation serves the high-resolution research needs for investigators from the University of Connecticut and nearby colleges and companies.

The NMR Facility directly supports and impacts research programs in the following areas:

  • Chemical synthesis
  • Analysis of Chemical Mixtures
  • Pharmaceutical chemistry
  • Nanomaterials
  • Molecular recognition & drug binding
  • Macromolecules
  • Protein structure-function relationships
  • Protein folding and design
  • Nucleic acid structure and reactivity
  • Molecular dynamics

Instrumentation

  • Varian INOVA 600 MHz: Fitted with a triple-resonance (1H, 13C, 15N) inverse cryogenic probe, the Varian 600 has a superior proton sensitivity (signal-to-noise ratio ~4500). Owing to a high proton sensitivity, the 600 is an instrument of choice for characterization of submicrogram quantities of soluble organic compounds and the natural products via the proton-detected experiments: 1H one-dimensional spectra, 1H-1H, 1H-13C, and 1H-15N two-dimensional correlation spectra (COSY, TOCSY, NOESY, HSQC, HMBC, etc). The spectrometer is also fully equipped to carry out the biomolecular multidimensional (3D, 4D) NMR experiments and Diffusion Ordered Spectroscopy (DOSY) experiments. The Varian 600 can be found in the Chemistry Building, room R002 (Rates).
  • Bruker AVANCE 500 MHz This walk-up spectrometer is equipped with the broadband 5 mm BBFO SmartProbe that can automatically select, optimally tune, and match with the auto tune-match (ATM) component to any nuclei in the resonance frequency range between 15N-31P. Besides, this BBFO probe has the added ability to observe 19F with 1H decoupling and vice-versa, as well as to perform 1H-19F 2D correlation spectroscopy. The probe has a signal-to-noise ratio 730 for 1H, 550 for 19F, 300 for 13C, 180 for 31P and 40 for 15N. The Bruker AVANCE 500 is an instrument of choice for users with small quantities of a compound and for multidimensional experiments such as HSQC, HMBC or NOESY. It is used when a higher resolution NMR is necessary for chemical structure elucidation and supports NMR studies on proteins and nucleic acids. The spectrometer is located in the Pharmacy/Biology Building, room 056 (Rates).
  • Bruker AVANCE III 400 MHz: This workhorse of the Chemistry Department is equipped with a two-channel console and a 5mm BBFO probe with 19F capability. It delivers a signal-to-noise ratio 300 for 1H, 275 for 19F, 190 for 13C, 150 for 31P, and 20 for 15N with automatic tuning and matching to selected nuclei. The AVANCE 400 is used for both one-dimensional and advanced two-dimensional experiments. It is located in the Chemistry Building, room R002 (Rates).
  • Bruker AVANCE 300 MHz: This two-channel instrument has a 5mm BBO probe with ATM component and can be used for a broad range of nuclei. It is mostly used for routine NMR spectroscopy and for undergraduate research and education (Rates).

In addition to the above NMR spectrometers, the Institute of Materials Science houses two Bruker NMR instruments: solid-state AVANCE III 400 MHz and the solution DMX 500 MHz. Visit the IMS NMR Laboratory for more information.

Training and Education

Only trained users can be granted an access to the NMR spectrometers. The NMR Facility Staff provides training and consultation in the use of instruments under their respective supervision. A 24-hour notice is required for scheduling a hands-on training session. For specific questions regarding applications for any NMR instrument and for any general managerial questions, please contact the NMR Facility Director Dr. Vitaliy Gorbatyuk at (860) 486-4069 or at Vitaliy.Gorbatyuk@uconn.edu.

NMR Service Rates: Varian INOVA 600 & Bruker AVANCE 500

Internal Users (any UConn faculty and members of their group)

Varian INOVA 600

  • $7.25/hr for unassisted use (i.e. users run their samples, after the NMR training session)
  • $27.25/hr for assisted use (i.e. NMR staff collects the data)
  • $75/hr Training/Set-up/Consulting fee

Bruker AVANCE 500

  • $6.25/hr for unassisted use
  • $26.25/hr for assisted use
  • $75/hr Training/Set-up/Consulting fee

External Users

Varian INOVA 600 and Bruker AVANCE 500

  • Academic Institutions (not-for-profit):
    • $50/hr for unassisted use (i.e. users run their samples, after the NMR training session)
    • $90/hr for assisted use (i.e. NMR staff collects the data)
    • $100/hr Training/Set-up/Consulting fee
  • Companies (for-profit):
    • $90/hr for unassisted use
    • $150/hr for assisted use
    • $150/hr Training/Set-up/Consulting fee

NMR Service Rates: Bruker AVANCE III 400 & AVANCE 300

Internal Users (any UConn faculty and members of their group)

Magnet time for AVANCE III 400 (i.e. users run their samples, after the NMR training session):

  • $6/hr in Primetime (8:00 AM to 8:00 PM)
  • $2/hr during Nighttime (8:00 PM to 8:00 AM), weekends, and holidays
  • $75/hr Training/Set-up/Consulting fee

Magnet time for AVANCE 300 (i.e. users run their samples, after the NMR training session):

  • $3/hr flat rate
  • $75/hr Training/Set-up/Consulting fee

External Users

Magnet time for Chemistry 300 and 400 (i.e. users run their samples, after the NMR training session):

  • Academic Institutions (not-for-profit):
    • $10/hr for unassisted use (i.e. users run their samples, after the NMR training session)
    • $90/hr for assisted use (i.e. NMR staff collects the data)
    • $100/hr Training/Set-up/Consulting fee
  • Companies (for-profit):
    • $50/hr for unassisted use
    • $150/hr for assisted use
    • $150/hr Training/Set-up/Consulting fee

Services done by the NMR Facility Manager

  • Not-for-profit:
    • $90/hr magnet time
    • $100/hr Set-up/Consulting fee
  • For-profit:
    • $150/hr magnet time
    • $150/hr Set-up/Consulting fee