Spundana Malla, Karteek Kadimisetty, You-Jun Fu, Dharamainder Choudhary,
John B. Schenkman & James F. Rusling
Methyl-Cytosine-Driven Structural Changes Enhance Adduction Kinetics of an Exon 7 fragment of the p53 Gene. Sci. Rep. 7, 40890; doi: 10.1038/srep40890 (2017).
In a collaborative effort with UConn Health (Profs. Schenkman and Choudhary), Spundana Malla, Karteek Kadimisetty, Dr. Fu and Prof. Rusling reported a new LC-MS method to investigate the kinetics of gene damage. The paper describes the use of the method to investigate metabolite-related damage to p53 tumor suppressor gene, which is damaged in 50% of all cancers. Results show that a specific cytosine-guanine site (codon 248) on the p53 gene reacts 3-fold faster when the cytosine is methylated.